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cryo em cleanup workflow  (Thermo Fisher)


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    Thermo Fisher cryo em cleanup workflow
    α1-containing cerebellar GABA A Rs show distinct α and β subunit <t>combinations.</t> <t>Cryo-EM</t> data analysis of the PZ-II-029/GABA dataset identifies five distinct receptor assemblies. ( A ) Two predominant assemblies with well-defined subunit identity, β2-α1-β2-α1-γ2 (viewed from the extracellular space, subunits counted counter-clockwise) and β2-α1-β1-α6-γ2. ( B ) Additional assemblies showing ambiguous density at one or both β subunit positions, corresponding to β2-α1-β2/3-α1-γ2, β1-α1-β1/2-α1-γ2, β1/2-α1-β2/3-α1-γ2. When two subunits are listed at one position, the first one denotes the predominant identity. Percentages of each receptor assembly are calculated based on the number of final particles used for the cryo-EM reconstruction ( SI Appendix , Fig. S5 ). The extracellular domain (ECD) is colored based on the subunit identity. All N -glycosylation is colored in teal, and representative glycosylation at the ECD periphery is labeled with an arrow.
    Cryo Em Cleanup Workflow, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryo+em+cleanup+workflow/SUCROSE+CRYSTAL+CERT+ACS+12KG/pmc12890884-43-34-34
    Average 98 stars, based on 1 article reviews
    cryo em cleanup workflow - by Bioz Stars, 2026-09
    98/100 stars

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    1) Product Images from "Molecular assemblies and pharmacology of cerebellar GABA A receptors"

    Article Title: Molecular assemblies and pharmacology of cerebellar GABA A receptors

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.2524504123

    α1-containing cerebellar GABA A Rs show distinct α and β subunit combinations. Cryo-EM data analysis of the PZ-II-029/GABA dataset identifies five distinct receptor assemblies. ( A ) Two predominant assemblies with well-defined subunit identity, β2-α1-β2-α1-γ2 (viewed from the extracellular space, subunits counted counter-clockwise) and β2-α1-β1-α6-γ2. ( B ) Additional assemblies showing ambiguous density at one or both β subunit positions, corresponding to β2-α1-β2/3-α1-γ2, β1-α1-β1/2-α1-γ2, β1/2-α1-β2/3-α1-γ2. When two subunits are listed at one position, the first one denotes the predominant identity. Percentages of each receptor assembly are calculated based on the number of final particles used for the cryo-EM reconstruction ( SI Appendix , Fig. S5 ). The extracellular domain (ECD) is colored based on the subunit identity. All N -glycosylation is colored in teal, and representative glycosylation at the ECD periphery is labeled with an arrow.
    Figure Legend Snippet: α1-containing cerebellar GABA A Rs show distinct α and β subunit combinations. Cryo-EM data analysis of the PZ-II-029/GABA dataset identifies five distinct receptor assemblies. ( A ) Two predominant assemblies with well-defined subunit identity, β2-α1-β2-α1-γ2 (viewed from the extracellular space, subunits counted counter-clockwise) and β2-α1-β1-α6-γ2. ( B ) Additional assemblies showing ambiguous density at one or both β subunit positions, corresponding to β2-α1-β2/3-α1-γ2, β1-α1-β1/2-α1-γ2, β1/2-α1-β2/3-α1-γ2. When two subunits are listed at one position, the first one denotes the predominant identity. Percentages of each receptor assembly are calculated based on the number of final particles used for the cryo-EM reconstruction ( SI Appendix , Fig. S5 ). The extracellular domain (ECD) is colored based on the subunit identity. All N -glycosylation is colored in teal, and representative glycosylation at the ECD periphery is labeled with an arrow.

    Techniques Used: Cryo-EM Sample Prep, Glycoproteomics, Labeling



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    cryo em cleanup workflow  (Thermo Fisher)
    98
    Thermo Fisher cryo em cleanup workflow
    α1-containing cerebellar GABA A Rs show distinct α and β subunit <t>combinations.</t> <t>Cryo-EM</t> data analysis of the PZ-II-029/GABA dataset identifies five distinct receptor assemblies. ( A ) Two predominant assemblies with well-defined subunit identity, β2-α1-β2-α1-γ2 (viewed from the extracellular space, subunits counted counter-clockwise) and β2-α1-β1-α6-γ2. ( B ) Additional assemblies showing ambiguous density at one or both β subunit positions, corresponding to β2-α1-β2/3-α1-γ2, β1-α1-β1/2-α1-γ2, β1/2-α1-β2/3-α1-γ2. When two subunits are listed at one position, the first one denotes the predominant identity. Percentages of each receptor assembly are calculated based on the number of final particles used for the cryo-EM reconstruction ( SI Appendix , Fig. S5 ). The extracellular domain (ECD) is colored based on the subunit identity. All N -glycosylation is colored in teal, and representative glycosylation at the ECD periphery is labeled with an arrow.
    Cryo Em Cleanup Workflow, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cryo+em+cleanup+workflow/SUCROSE+CRYSTAL+CERT+ACS+12KG/pmc12890884-43-34-34
    Average 98 stars, based on 1 article reviews
    cryo em cleanup workflow - by Bioz Stars, 2026-09
    98/100 stars
    		  Buy from Supplier

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    α1-containing cerebellar GABA A Rs show distinct α and β subunit combinations. Cryo-EM data analysis of the PZ-II-029/GABA dataset identifies five distinct receptor assemblies. ( A ) Two predominant assemblies with well-defined subunit identity, β2-α1-β2-α1-γ2 (viewed from the extracellular space, subunits counted counter-clockwise) and β2-α1-β1-α6-γ2. ( B ) Additional assemblies showing ambiguous density at one or both β subunit positions, corresponding to β2-α1-β2/3-α1-γ2, β1-α1-β1/2-α1-γ2, β1/2-α1-β2/3-α1-γ2. When two subunits are listed at one position, the first one denotes the predominant identity. Percentages of each receptor assembly are calculated based on the number of final particles used for the cryo-EM reconstruction ( SI Appendix , Fig. S5 ). The extracellular domain (ECD) is colored based on the subunit identity. All N -glycosylation is colored in teal, and representative glycosylation at the ECD periphery is labeled with an arrow.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Molecular assemblies and pharmacology of cerebellar GABA A receptors

    doi: 10.1073/pnas.2524504123

    Figure Lengend Snippet: α1-containing cerebellar GABA A Rs show distinct α and β subunit combinations. Cryo-EM data analysis of the PZ-II-029/GABA dataset identifies five distinct receptor assemblies. ( A ) Two predominant assemblies with well-defined subunit identity, β2-α1-β2-α1-γ2 (viewed from the extracellular space, subunits counted counter-clockwise) and β2-α1-β1-α6-γ2. ( B ) Additional assemblies showing ambiguous density at one or both β subunit positions, corresponding to β2-α1-β2/3-α1-γ2, β1-α1-β1/2-α1-γ2, β1/2-α1-β2/3-α1-γ2. When two subunits are listed at one position, the first one denotes the predominant identity. Percentages of each receptor assembly are calculated based on the number of final particles used for the cryo-EM reconstruction ( SI Appendix , Fig. S5 ). The extracellular domain (ECD) is colored based on the subunit identity. All N -glycosylation is colored in teal, and representative glycosylation at the ECD periphery is labeled with an arrow.

    Article Snippet: In data analysis for both datasets ( SI Appendix , Figs. S3–S5 ), more than 2 million GABA A R particles exhibiting salient receptor features in 2D class averages are obtained following a simple cryo-EM cleanup workflow ( SI Appendix , Figs. S3 A and S5 A ).

    Techniques: Cryo-EM Sample Prep, Glycoproteomics, Labeling